What are the techniques used in Immunodetection?

  • Thread starter soundlover
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In summary, Immunodetection is the use of labeled antibodies to detect proteins, commonly applied in techniques such as western blots, ELISA, and immunocytochemistry/immunohistochemistry. It is also used in home pregnancy tests. Some labs are transitioning from using radioactive materials in techniques such as RIA to using ELISA.
  • #1
soundlover
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lo all,first post here,this seems to be a nice forum:)

i´m trying to know a litle more about Immunodetection,how does it work,witch techniques are applied,etc.
thanks in advance for any info,responses,links,etc :smile:
take care
 
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  • #2
Immunodetertion is the use of labeled antibodies for detecting proteins. The antibodies can be labeled with radioactive isotopes or with enzyme/chemicals that combine to specifics chemicals will give a color (colorimetric test) or will fluoresce (fluorescence test).

As far as the application goes, there is a huge list of it but the most common is the home pregnancy test kit. This is a colorimetric test.

The more common list of techniques used in research lab are western blots and ELISA.
 
  • #3
Also included as techniques used are immunocytochemistry/immunohistochemistry and I'm one of the last generation to have learned the dying art of radioimmunoassay (RIA), which many labs are changing over to using ELISA in order to reduce the amount of radioactive materials being used.

And welcome to PF! :smile:
 

1. What is immunodetection and how does it work?

Immunodetection is a technique used to identify and measure specific proteins or molecules in a sample. It works by using antibodies, which are proteins that bind to specific targets, to detect the presence of the molecule of interest. The antibodies are typically labeled with a fluorescent or enzymatic marker, allowing for visualization or quantification of the target.

2. What are the different types of immunodetection techniques?

There are several types of immunodetection techniques, including western blotting, enzyme-linked immunosorbent assay (ELISA), immunofluorescence, and immunohistochemistry. Each of these techniques utilizes antibodies in different ways to detect and measure specific molecules in a sample.

3. What are the advantages of using immunodetection techniques?

One of the main advantages of immunodetection techniques is their specificity and sensitivity. This means that they can accurately detect and measure very small amounts of a specific target molecule in a complex sample. Additionally, these techniques are relatively easy to perform and can be adapted for use in a variety of sample types.

4. What are some common challenges in using immunodetection techniques?

One of the main challenges in using immunodetection techniques is ensuring the specificity of the results. Cross-reactivity with other molecules can lead to false positive results. Another challenge is optimizing the assay conditions and antibody concentrations to ensure accurate and reproducible results.

5. How are immunodetection techniques used in research and medical diagnostics?

Immunodetection techniques are widely used in both research and medical diagnostics. In research, they are used to study the expression and function of specific molecules in cells and tissues. In medical diagnostics, these techniques are used to detect and measure biomarkers for various diseases and conditions, such as cancer and infectious diseases.

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