Interactions with the mobile phase

In summary, the chemical interactions between the mobile phase and sample, as well as the interactions with the column, determine the migration and separation of components in a sample. The term "reverse" refers to inverting the variables discussed, such as stronger interactions with the stationary phase instead of the mobile phase leading to faster elution and shorter retention time. This type of thinking may be challenging for some, but it is important to understand in order to fully comprehend the topic.
  • #1
mountain
53
0
from my book it says:

The chemical interactions of the mobile phase and sample, with the column, determine the degree of migration and separation of components contained in the sample. For example, those samples which have stronger interactions with the mobile phase than with the stationary phase will elute from the column faster and thus have a shorter retention time, while the reverse is also true.

what do they mean when they say "while the reverse is also true." ? :yuck:

hope very much for ideas!

thanks!
 
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  • #2
mountain said:
--- stronger interactions with the mobile (reverse of "mobile" = stationary) phase than with the stationary (and, --- fill in the blank) phase will elute from the column faster (reverse is ?) and thus have a shorter (reverse is ?) retention time ---

"Reverse" means invert the variables that have been discussed.
 
  • #3
Bystander said:
"Reverse" means invert the variables that have been discussed.

hehe...you are smart! i never thought the author would think this way. geeze, i just hate when they tease my brain :grumpy: thanks alot! :smile:
 

1. What is the mobile phase in chromatography?

The mobile phase is the liquid or gas that carries the sample through the chromatography system. It moves through the stationary phase, which contains the substance that separates the components of the sample.

2. How does the mobile phase affect the separation of compounds?

The composition and properties of the mobile phase, such as its polarity and flow rate, can impact the separation of compounds in chromatography. A more polar mobile phase may result in better separation of polar compounds, while a higher flow rate can decrease resolution between compounds.

3. What is the role of the mobile phase in thin-layer chromatography?

In thin-layer chromatography, the mobile phase is responsible for carrying the sample along the stationary phase, which is a thin layer of absorbent material on a solid support. The mobile phase moves up the plate by capillary action, causing the components of the sample to separate based on their affinity for the stationary phase.

4. Can the mobile phase be changed during a chromatography experiment?

Yes, the mobile phase can be changed during a chromatography experiment to optimize separation of compounds. However, changing the mobile phase may also alter the separation pattern and should only be done if necessary.

5. How is the mobile phase chosen for a specific chromatography experiment?

The choice of mobile phase depends on the type of chromatography being performed and the compounds being separated. Factors such as solubility, polarity, and molecular weight of the sample should be considered when selecting the appropriate mobile phase.

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