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Ka of PBS

  1. Apr 26, 2007 #1
    Anyone know the Ka for Phosphate Buffered Saline (pH 7.4). I have heard that it is 6.2E-8, but the formula says .008M Na2HPO4, .002M KH2PO4. Using Henderson Haselbalch, [H+] = Ka[KH2PO4]/[Na2HPO4] using pH of 7.4, Ka = 1.59E-7. Which one is correct?
     
  2. jcsd
  3. Apr 26, 2007 #2

    Borek

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    Staff: Mentor

    pKas - 2.148, 7.199, 12.35. You should take solution ionic strength into account before calculations.
     
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