(adsbygoogle = window.adsbygoogle || []).push({}); 1. The problem statement, all variables and given/known data

A monovalent ligand binds to a protein with six in independent, identical binding sites. What is the probability that a given protein molecule is bound by at least five ligand molecules at equilibrium if K[tex]^{\mu}_{D}[/tex] = 1nM and L[tex]_{0}[/tex]=2nM (constant)?

2. Relevant equations

I don't really know what equations to use to get started on this.

3. The attempt at a solution

I suppose this would be more of a probability or statistics based problem, but I have to take into consideration the protein binding affinity and ligand concentration. I know K[tex]_{D}[/tex] is K[tex]_{off}[/tex]/K[tex]_{on}[/tex], so that would be a measure of the probability of binding to any one spot. The initial ligand concentration also determines the probability of binding since it allows for more ligand to be bound to the protein binding sites. But I don't know how to use these definitions to create a mathematical way to find the actual numerical probability.

I would appreciate any help.

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# Homework Help: Ligand-protein binding

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