I will be attempting to ligate a gene fragment (~650bp's) into a Pbluescript plasmid here in the next day or so. The fragment and the vector have both already been digested and purified, so that's not an issue. However, I have been advised by a number of differnt people that the concentrations of my gene relative to my vector are VERY important and I do not know what this relationship is supposed to be. I looked in the Short Protocols Molecular Biology and did not find anything. Does anyone have any good links or words of wisdom? Thanks!