lysis buffer- help! the protocol for the lysis buffer i use is: 5mM Tris 1mM EDTA pH7.4 does it mean that the lysis buffer has to be pH7.4 or does it mean that the EDTA buffer alone is ph7.4? can you guys tell me which one of these options is correct? 1. add EDTA and Tris to the same bottle with the correct amounts and adjust the pH to 7.4. the lysis buffer will be pH7.4 2. make a stock solution of EDTA pH7.4 and add the right volume to the Tris solution. the lysis buffer won't be pH7.4 (due to the Tris) i appreciate for any ideas! thanks alot!