Making 50%v/v and 50%w/v glycerol solutions.

  • Thread starter sobored
  • Start date
In summary, to make a 50% v/v glycerol solution, you would need to measure out 50 mL of glycerol and add it to 50 mL of water. To make a 50% w/v glycerol solution, you would need to measure out 50 g of glycerol and add it to 100 mL of water. The main difference between the two is the method of measurement, with the v/v solution measuring by volume and the w/v solution measuring by weight. Additionally, the density of the solutions may vary, with the v/v solution potentially containing more glycerol than the w/v solution.
  • #1
sobored
52
0
How to make 50%v/v and 50%w/v glycerol? What is the difference? Glycerol/glycerin has a density of 1.25 gm/mL.



Thanks!
 
Chemistry news on Phys.org
  • #2
I guess you are making frozen stock of cells.

Glycerol usually comes in a liquid form. So 50% v/v would be 50 mL of glycerol in a 100 mL solution. A 50% w/v would be 50 g per 100 mL of solution. So basicly you measure your glycerol by weight (50 g) or by volume (50 mL), depending on your solution, then raise the volume to 100 mL with ddH20.

So if you known the density, all you have to do is calculate the amount of g in a 50% v/v solution and compare it to your 50% w/v solution. You can also calculate the density of both solution and compare it. Basicly one solution has more glycerol than the other one.
 
Last edited:
  • #3
Making 50%v/v and 50%w/v glycerol solutions can be done by following these steps:

1. For a 50%v/v solution: Measure out 50 mL of glycerol and add it to a 100 mL graduated cylinder. Then, add enough water to bring the total volume up to 100 mL. This will give you a 50%v/v glycerol solution, meaning that 50 mL of the solution is made up of glycerol.

2. For a 50%w/v solution: Measure out 50 grams of glycerol and add it to a 100 mL beaker. Then, add enough water to bring the total volume up to 100 mL. This will give you a 50%w/v glycerol solution, meaning that 50 grams of the solution is made up of glycerol.

The difference between these two solutions is the unit of measurement used. The v/v (volume/volume) solution is expressed as a percentage of the total volume, while the w/v (weight/volume) solution is expressed as a percentage of the total weight. This is because glycerol has a density of 1.25 gm/mL, so 50 mL of solution would weigh more than 50 grams.

It's important to note that the final volume of the solution may not exactly be 100 mL due to the slight difference in densities between glycerol and water. However, it should be close enough for practical purposes.

I hope this helps! Let me know if you have any further questions.
 

What is the difference between a 50%v/v and 50%w/v glycerol solution?

A 50%v/v glycerol solution means that 50% of the final solution is made up of glycerol by volume. This means that if you have a total of 100 mL of solution, 50 mL will be glycerol and the remaining 50 mL will be made up of other substances. On the other hand, a 50%w/v glycerol solution means that 50% of the final solution is made up of glycerol by weight. This means that if you have a total of 100 g of solution, 50 g will be glycerol and the remaining 50 g will be made up of other substances.

Why is it important to accurately measure the concentration of glycerol in a solution?

Glycerol is commonly used as a solvent or preservative in many laboratory experiments and processes. Accurate measurement of its concentration is important because it can affect the outcome of the experiment or process. Too much or too little glycerol can alter the properties of the solution and lead to incorrect results.

What is the best method for preparing a 50%v/v or 50%w/v glycerol solution?

The best method for preparing a 50%v/v or 50%w/v glycerol solution is to use a volumetric flask and a balance. First, measure out the desired volume or weight of glycerol using a graduated cylinder or pipette. Then, add it to the volumetric flask and fill it up to the mark with the appropriate solvent, either water for a v/v solution or another substance for a w/v solution. Finally, mix well to ensure the glycerol is evenly distributed throughout the solution.

What are the potential hazards of working with glycerol?

Glycerol is generally considered safe to handle, but it can cause irritation to the skin and eyes. It is also flammable, so care should be taken when using it near heat sources. Inhaling high concentrations of glycerol can also cause respiratory irritation. It is important to wear proper protective equipment, such as gloves and goggles, when working with glycerol.

Can a 50%v/v or 50%w/v glycerol solution be stored for long periods of time?

Glycerol solutions can be stored for long periods of time, but it is important to keep them in a tightly sealed container and away from heat and light. This will prevent evaporation and degradation of the glycerol. It is also recommended to periodically check the concentration of the solution to ensure it has not changed over time.

Similar threads

Can glycerol do this in the body?
    • Biology and Medical
Replies
2
Views
3K
Chemical Polymer Separation (PLA, ABS, HIPS, and PETG)
    • Chemistry
Replies
3
Views
2K
Calculating viscosity of a liquid with a falling object
    • Biology and Chemistry Homework Help
Replies
4
Views
2K
Aqueous solution of tolyltriazole
    • Chemistry
Replies
3
Views
865
Help with PVP-I & DMSO solution
    • Chemistry
Replies
2
Views
3K
Given specific v, dimension of subspace of L(V, W) where Tv=0?
    • Calculus and Beyond Homework Help
Replies
14
Views
582
TiO2 Photocatalyst to replicate making a photocatalytic PVA film
    • Chemistry
Replies
1
Views
923
Thermodynamics relating to temperature and phase changes V.2
    • Introductory Physics Homework Help
Replies
1
Views
1K
Making a handcranked flashlight with a supercapcitor
    • Engineering and Comp Sci Homework Help
Replies
7
Views
202
Find a basis for W which is subset of V
    • Calculus and Beyond Homework Help
Replies
4
Views
654

Hot Threads

Recent Insights

Back
Top