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Making protein prep solutions

  1. Aug 22, 2005 #1
    I have searched and searched till my eyes fall out, but can not find any recipes for making these solutions. :grumpy: Anyone can help me out?

    1mM tributylphosphine
    1%SDS
    10 M Urea
     
  2. jcsd
  3. Aug 22, 2005 #2

    iansmith

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    It mostly a matter of you calculating the weight required for 1 mM solution and a 10 M solution. Urea is solid and Tributylphosphine is usually in liquid. Both should be found in your lab. It is basic chemistry.

    As far the SDS solution goes, it is probably w/v but it might be specifiy. So when dealing with percentages in a recipe in term of w/v, it is about an amount of grams per 100 mL of solution. Therefore, it would be 1 g of SDS per 100 mL of solution.
     
  4. Aug 22, 2005 #3
    hI iAN,

    I am thinking of the solvent (?) to dissolve these stuffs. For example for SDS solution, should i use sterile water or buffers?
     
  5. Aug 22, 2005 #4

    iansmith

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    SDS and Urea can be diluted into distilled deionized water. Usually, if a chemical solution requires to be prepare in a buffer, it should be mention in the name of solution.

    For tributylphosphine, I am not sure but sigma keeps their 200 mM solution in N-methyl-2-pyrrolidine.
    http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/T7567

    You may want to check this reference given by sigma.
    Herbert, B., et al., Reduction and alkylation of proteins in preparation of two dimensional map analysis: why, when, and how?. Electrophoresis 22, 2046-57, (2001)
     
  6. Aug 23, 2005 #5

    Moonbear

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    Whenever you're in doubt, check the spec sheet that comes with the chemicals for information on solubility. If the chemicals didn't come with a spec sheet and you aren't sure, contact the manufacturer for solubility information.
     
  7. Aug 23, 2005 #6
    if you are doing protein chemistry you should really use nanopure water.

    % sds is almost always w/v
     
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