# Microscope magnifications

1. Dec 18, 2009

### agentas

1. The problem statement, all variables and given/known data

I had a lab. where ´the compound microscope was used.The microscope was equipped with 4X,10X,20X,40X,100X objectives and oculal 10X.First I had to calibrate my microscope using 1mm long calibrated graticule (divided in 100 grid lines-distance between two lines is 10 micrometres) for all those different magnification.I did this finding that for total magnification of 40 the ocular (also divided in 100 lines) distance in oculur is 5 mircometres:
for 100 - 2 micrometres
200 - 1 micrometre
400 - 0,5 micrometre
My question is how I can measure now real experimental magnification,because my teacher said what I have measured now is nominal magnification and real measured magnifications are different.Please help me I can not figure out.

2. Relevant equations

M(total)=m(objective) x m(ocular)
M=image/object

3. The attempt at a solution
if I divide 5 micrometres by 10 micrometres I will get 0,5 magnification.What does not make sense.I do not understand how to calculate experimental magnification.Please help!!!
if I divide