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Biology and Chemistry Homework Help
Molar extinction coefficient calculation.
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[QUOTE="Lahe, post: 4519780, member: 489493"] [h2]Homework Statement [/h2] I need to calculate molar extinction coefficient for Albumin at 280nm. [h2]Homework Equations[/h2] A=ε*c*L ε=A/(c*L) But since L=constant, I can just say ε=A/c [h2]The Attempt at a Solution[/h2] The given molecular mass of albumin is 66,5kDa, which equals 66500g/mol. I do understand that I can find c (concentration) via the molecular mass. What I don't understand is how can I find ε if I still have another unknown, which in this case happens to be A. So: ε(unknown)=A(unknown)/c(known). Have I missed something really simple and overthinking this for myself? How can I find Absorbance? [/QUOTE]
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Biology and Chemistry Homework Help
Molar extinction coefficient calculation.
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