Post any questions you have about Molecular Biology in here (or anything related...Microbiology, lab techniques, experiments etc). Whether you want to know something specific (What is a nucleotide? for example), or something broad (What are RFLP's? for example). This thread could be useful to everyone whether you are a Molecular Biology student, or just someone who has read an article that refered to some terminology you didn't understand. Ask here and Monique, Ian, myself, or any one of the many other resident 'know it mosts' will try to answer it. So, to start it off: I'm curious to find out more about PCR Primer design. Can anyone fill me in some more about Star Activity and annealing temperatures etc.