You can generally input data from a file into a simulation, operate on it, then capture that data to a signal and write it to a file. You can then convert it to whatever format you choose.
The actual commands to do that depends on the simulator, but it is basically learning to make use of the various $read and $write simulator functions.
Not sure what multisim has available, if anything.
For example the following (from an ncsim file, not multisim)
$fwrite(tester_vectorsFPGA_filehandle,"%s %s %b %b //%0d\n",dpstringFPGA, dmstringFPGA, txoe, testeroe, vector_num12);
will write to the file represented by the filehandle: two strings (contained in dpstringFPGA, dmstringFPGA) , and 2 binary values (contained in txoe, testeroe) and a decimal value vector_num12
You just capture the data into a variable at each clock edge, and write it out.
You do a similar thing to read a file into a 2 dimensional array, then iterate through the array on each clock edge (or as you need the data).
Generally the array needs to be large enough to hold the whole file.