Dear everybody, Please help me out. I am doing an experiment where i use B actin as the normalizer. The problem is after i have stripped the filters then some of the wells did not have the B actin signals. Even if the intensity of the target protein in that well had the same strength as the others, so this could not be a loading problem, could it? What is wrong? I bought the mouse monoclonal antibody against human B actin from Santa Cruz Biotechnology, Inc. Has anyone used this before? I used 500X dilution. Should i reduce the dilution factor? Do you know any well known manufacturers for selling good antibody against human B actin? Thanks!