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Orientation factor in Resonance Energy Transfer

  1. Dec 22, 2012 #1

    I'm currently stuck on how to derive the orientation factor for RET. I've searched a lot of journals articles on how to derive it and I find their derivations to be hand wavy. Basically, the common equations for RET are:

    κ2 = (cosθT - 3cosθDcosθA)2 = (sinθDsin θAcosΦ - 2cosθDcosθA)2

    I've also attached a diagram associated with it:
    Source: http://www.olympusfluoview.com/applications/fretintro.html [Broken]

    Can somebody point me to the right direction on how to derive this? It isn't simple geometry in my opinion.
    Last edited by a moderator: May 6, 2017
  2. jcsd
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