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Dr. Nick
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Point of experiment was to see physiological role and influence of ACTH and Dexamethasone on adrenal gland via cholesterol extracted from gland. And we invested glucose level in blood after treatment.
Cholesterol results were as expected, Dex treated animals had the highest amount of cholesterol in glands, then control group, at the and ACTH group.
But we have the problem with glucose reading. DEX treated group is OK, they have higher than control glucose level.
ACTH treated should have higher glucose level in blood than control group, but they have almost the same glucose concentration.
Now my problem: we have to explain this unusual result. But we have to explain it from physiological point of view, I mean we have to give physiological reasons for this unexpected result. Even dough it was probably mistake done during experiment.
Can you help me with probable reasons for this. Professor sad that we should try to find science papers that explains this result for glucose, and than quote them in our paper. But no luck, we've found none. Please help, THANK YOU!
p.s. I was thinking that ACTH treatment was to late to induce corticosteroids release into blood or there was to little time for corticosteroids to induce glucose release into the blood. But I can't back up this idea with some science paper. We've treated them 3h before sacrifice with 6µg ACTH.
Glucose results:
http://img156.imageshack.us/img156/8937/glucoserf4.jpg
p.s.s. you don't allow tags, strange, they are useful, and especially could be useful for biology forum
Cholesterol results were as expected, Dex treated animals had the highest amount of cholesterol in glands, then control group, at the and ACTH group.
But we have the problem with glucose reading. DEX treated group is OK, they have higher than control glucose level.
ACTH treated should have higher glucose level in blood than control group, but they have almost the same glucose concentration.
Now my problem: we have to explain this unusual result. But we have to explain it from physiological point of view, I mean we have to give physiological reasons for this unexpected result. Even dough it was probably mistake done during experiment.
Can you help me with probable reasons for this. Professor sad that we should try to find science papers that explains this result for glucose, and than quote them in our paper. But no luck, we've found none. Please help, THANK YOU!
p.s. I was thinking that ACTH treatment was to late to induce corticosteroids release into blood or there was to little time for corticosteroids to induce glucose release into the blood. But I can't back up this idea with some science paper. We've treated them 3h before sacrifice with 6µg ACTH.
Glucose results:
http://img156.imageshack.us/img156/8937/glucoserf4.jpg
p.s.s. you don't allow tags, strange, they are useful, and especially could be useful for biology forum
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