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Pleeeease help, FISH&friends

  1. Jul 27, 2005 #1
    I’ve really lost my nerves on this one :(, I have to learn some methods and differences between them, but I can’t manage it. And all I have is an internet as help.

    Methods are :
    Chromosome painting

    I just can’t establish difference between some of them, purposes.
    Especially Chromosome painting, c’mon I don’t get it, one site says it’s basically mFISH, on other something else. :confused:

    This is where I am now:

    FISH - using singlestrain DNA fluorescence labeled probe (1 probe I guess)
    mFISH - using multiple singlestrain DNA fluorescence labeled probes giving different colours
    Chromosome painting – same as mFISH ????
    BandFISH – painting whole chromosome in different color bands
    SKY – painting all chromosomes (with what ?, DNA probes ?) in different colors for cariotyping

    And when in FISH picture you see red probe on chromosome you can see whole chromosome painted in blue, is this done by probes of some kind of color specificlu binding for chromosomes (I’ve saw this in other methods too).

    Thank You !!!
  2. jcsd
  3. Jul 27, 2005 #2
    Situation is better now, once I found better resources on the net…

    p.s. ARMS = ASO ?
  4. Jul 28, 2005 #3
    AS PCR is also known as ARMS
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