Pleeeease help, FISH&friends

  • Thread starter Dr. Nick
  • Start date
  • #1
28
0

Main Question or Discussion Point

I’ve really lost my nerves on this one :(, I have to learn some methods and differences between them, but I can’t manage it. And all I have is an internet as help.

Methods are :
FISH
mFISH
Chromosome painting
BandFISH
SKY

I just can’t establish difference between some of them, purposes.
Especially Chromosome painting, c’mon I don’t get it, one site says it’s basically mFISH, on other something else. :confused:

This is where I am now:

FISH - using singlestrain DNA fluorescence labeled probe (1 probe I guess)
mFISH - using multiple singlestrain DNA fluorescence labeled probes giving different colours
Chromosome painting – same as mFISH ????
BandFISH – painting whole chromosome in different color bands
SKY – painting all chromosomes (with what ?, DNA probes ?) in different colors for cariotyping

And when in FISH picture you see red probe on chromosome you can see whole chromosome painted in blue, is this done by probes of some kind of color specificlu binding for chromosomes (I’ve saw this in other methods too).

Thank You !!!
 

Answers and Replies

  • #2
28
0
Situation is better now, once I found better resources on the net…

p.s. ARMS = ASO ?
 
  • #3
28
0
Update:
AS PCR is also known as ARMS
 

Related Threads on Pleeeease help, FISH&friends

Replies
9
Views
8K
Replies
4
Views
5K
  • Last Post
Replies
7
Views
3K
  • Last Post
Replies
6
Views
3K
  • Last Post
Replies
16
Views
5K
  • Last Post
Replies
19
Views
9K
  • Last Post
Replies
1
Views
2K
  • Last Post
Replies
9
Views
47K
  • Last Post
Replies
16
Views
17K
  • Last Post
Replies
8
Views
4K
Top