Discovering a Protein's Primary Structure: Methods and Strategies

  • Thread starter mather
  • Start date
  • Tags
    Structure
In summary, if you do not have a peptide to work with, or you cannot crystalize it, you cannot predict its sequence. Otherwise, the three methods of predicting sequence that are mentioned on the linked page are available.
  • #1
mather
146
0
hello!

what are the available methods to determine the sequence (primary structure) of a protein?

from where one can start (what info he can gather) and how he can deduce the primary structure?

thanks!
 
Chemistry news on Phys.org
  • #3
sorry, not even close to what I am asking
 
  • #4
Perhaps if you could explain a bit more in detail what you need, it could be helpful.

The previously mentioned site notes that the advent of large amounts of genomic information has made it possible to predict protein sequences based on the corresponding nucleotie sequence. Also, mass spectrometry has been tremendously helpful in this area - see, for example, this perspective/review article.
 
  • #5
if I give you the volume of the peptide (let's only talk about small peptides), the charge/polarity/hydrophobicity of the various parts of its surface, its precise dimensions, will you be able to predict its sequence?

if not, why?
if not, is there anything else you would need to do it?
 
  • #6
If we say that there are 20 common amino acids, and only 20, and that they always link in the same way (e.g. no gamma-gln linkages) then there are 3.2 million pentapeptides. There is only one possible way that you can use the physical and/or chemical properties to identify anyone of those pentapeptides in an intact molecule: prepare a suitable crystal of the material, and determine its crystal structure by X-ray diffraction (minimum of a month of research if you are familiar with the techniques and fortunate enough to find a way to prepare the crystal). Otherwise, destructive techniques are always necessary. Obviously those that require only a small amount of material are preferable. The Edman degradation does lend itself to automation.

Sequencing methods that are used in practice are just exactly those that are described in the U Wisconsin article linked above.
 
  • #7
I don't have the protein or I cannot crystalize it

is there a workaround?

can I "generate computationally" a crystal, given its physical/chemical properties (which is what I only know) and then computationally again hit it with x-ray and determine its sequence?
 
  • #8
OK, the way your original post seems to read is "Suppose I have here a sample of a protein. How could I find out its sequence?"

It is now sounding like you are asking something a little different.

The X-ray diffraction technique depends on measuring the angular position and intensity of x-ray "spots" that you can find with a photon detector when you shine a beam of X-rays onto a small crystal of a substance.

If you do not know the sequence of a peptide, there is no way of telling where these spots will be theoretically. In fact, to predict the positions of the diffraction spots you would also need to know the secondary structure, and the way that a large number of molecules could make a regular packing.

If you do not have the peptide, there is no way that you could say accurately enough what any of its physical or chemical properties might be anyway.

So are you trying to identify a small peptide that you have a sample of? Or design a small peptide to have a particular physical or chemical property? Or to work out what some of the physical or chemical properties of a particular small peptide with a known sequence?
 
  • #9
mather said:
sorry, not even close to what I am asking

Uh, what? You asked about methods of determining primary structure. I gave you a link with three distinct methods of doing just that. If you're changing your question, you could have just clarified and said that instead of implying my reading comprehension sucks when your initial question was clear as crystal.
 
  • #10
JohnRC said:
So are you trying to identify a small peptide that you have a sample of?

no, since I don't have sample of the peptide (I just know some of its physical/chemical properties, and maybe some parts of its sequence or maybe that some specific aminoacids are not present in the sequence, etc)

JohnRC said:
Or design a small peptide to have a particular physical or chemical property? Or to work out what some of the physical or chemical properties of a particular small peptide with a known sequence?

yes this is my quest basically:

design [try to find possible/compatible sequences of] a small peptide to have a particular physical or chemical property

I bet it's hard and not very accurate, but I would like to know the current abilities we have regarding this

JohnRC said:
Or to work out what some of the physical or chemical properties of a particular small peptide with a known sequence?

no, I know [some of] it's physical/chemical properties, its sequence is what I don't know

ofcourse, if finding some specific physical/chemical properties will lead me to elucidate its sequence, it would be interesting
 
  • #11
aroc91 said:
Uh, what? You asked about methods of determining primary structure. I gave you a link with three distinct methods of doing just that. If you're changing your question, you could have just clarified and said that instead of implying my reading comprehension sucks when your initial question was clear as crystal.

no bad feelings, let's agree what I was asking was:
JohnRC said:
a little different
 
  • #12
Fair enough. It just seemed like a bit of a blunt response.
 
  • #13
mather said:
yes this is my quest basically:

design [try to find possible/compatible sequences of] a small peptide to have a particular physical or chemical property

I bet it's hard and not very accurate, but I would like to know the current abilities we have regarding this

Perhaps you're looking for something like this?
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1538902/
 
  • #14

1. What is the primary structure of a protein?

The primary structure of a protein refers to the specific sequence of amino acids that make up the protein. This sequence is determined by the genetic code of an organism and is essential for determining the protein's function and structure.

2. How is the primary structure of a protein determined?

The primary structure of a protein can be determined through various methods, including protein sequencing techniques such as Edman degradation, mass spectrometry, and DNA sequencing. These methods allow for the identification and sequencing of the individual amino acids in a protein.

3. What are the benefits of knowing a protein's primary structure?

Knowing a protein's primary structure is crucial for understanding its function and role in biological processes. It can also aid in the development of treatments for diseases, as well as in the design of new proteins for various purposes.

4. What are some common strategies used for discovering a protein's primary structure?

Some common strategies for discovering a protein's primary structure include isolating and purifying the protein, using various sequencing techniques, and comparing the protein to known sequences in databases. Additionally, computer modeling and prediction methods can also be used to determine a protein's primary structure.

5. Are there any limitations or challenges in determining a protein's primary structure?

Yes, there are limitations and challenges in determining a protein's primary structure. These include the complexity and size of the protein, as well as potential errors in sequencing methods. Additionally, some proteins may have modifications or alternate forms, making it difficult to determine their complete primary structure.

Similar threads

Replies
5
Views
1K
  • Biology and Medical
Replies
5
Views
742
Replies
1
Views
754
Replies
7
Views
2K
  • Biology and Medical
Replies
3
Views
2K
  • Biology and Medical
2
Replies
50
Views
9K
Replies
3
Views
2K
Back
Top