Quantum yield: fluorescence spectrum correction

[amattiol]

Hello,
I hope all is well, I have a few questions regarding fluorescence spectral corrections. I have run into an issue with corrections on the instrument I'm using. I have obtained spectra for a few compounds where the correction was working properly and achieve the results as expected. When I shift the emission scanning range down to around 280nm to 450nm, the correction file does not initiate - an error tells me this. This results in a very low intensity spectrum which in turn can not be used for quantum yield determinations by the reference method; I believe this is correct.

I wasn't aware of this issue until recently, is there a way to correct these spectra after the fact?
Any help would be greatly appreciated.

 

[jbsteele]

Unfortunately, there is no straightforward way to correct fluorescence spectra after the fact. You may be able to use a combination of software tools and manual data adjustment techniques to make some corrections, but this is not necessarily reliable. The best way to ensure accurate spectral corrections is to make sure the correction file is working properly when taking the measurements. If you are unable to get it to work properly in the lower range, you may need to switch to a different instrument or use a different method for collecting the data.