Quantum yield: fluorescence spectrum correction

  1. Hello,
    I hope all is well, I have a few questions regarding fluorescence spectral corrections. I have run into an issue with corrections on the instrument I'm using. I have obtained spectra for a few compounds where the correction was working properly and achieve the results as expected. When I shift the emission scanning range down to around 280nm to 450nm, the correction file does not initiate - an error tells me this. This results in a very low intensity spectrum which in turn can not be used for quantum yield determinations by the reference method; I believe this is correct.

    I wasn't aware of this issue until recently, is there a way to correct these spectra after the fact?
    Any help would be greatly appreciated.
  2. jcsd
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