Raman Microscopy

[n0_3sc]

Main Question or Discussion Point

What's the best way to hold a sample of micron-sized polystyrene beads for raman microscopy?

 

[Andy Resnick]

With a laser tweezer:

Raman spectroscopy of optically trapped particles
D V Petrov 2007 J. Opt. A: Pure Appl. Opt. 9 S139-S156 doi: 10.1088/1464-4258/9/8/S06

 

[n0_3sc]

Already know about that...seems I'm going to have to use microscope slides...

 

[n0_3sc]

After reading that article and other articles regarding optical traps and microscopy, it seems that the optical trapping is an automatic process? Ie. you still need the cover slides but the particles are automatically trapped by the beam.

"Optical Trapping and Coherent Anti-Stokes Raman Scattering (CARS) Spectroscopy of Submicron-Size Particles"
IEEE JoQE Vol.11 No. 4 Jul/Aug 2005