Rate constant of enzymatic reaction

1. Oct 25, 2003

schordinger

Hi everyone,

I am now doing a final year project about a special kind of beta-lactamase analysis. I am able to get the binding equilibrium constant, however, I have some difficult in finding out the rate constant of the binding of antibioatic and this enzyme.
It is because the my lab have no some advance instrument like microflow system. Indeed, all the method discussed in journal (in this few years) require those microflow system.....
the older method, like using dialysis tubing would be too "expensive" because the enzyme I used could not be synthesized easily....(usually I use 10~50 micro-lite in each set of data)

Thus, I would like to ask is there any simple method which require only small amount enzyme (<50ul) ???
Is that is impossible??

Thx.....
thx very much...