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Reducing or not!

  1. Sep 23, 2004 #1
    what is the difference between a non-reducing and a reducing buffer? :uhh:

    and what does it mean with non-reducing and reducing?

    thanks a bunch!
    Last edited: Sep 23, 2004
  2. jcsd
  3. Sep 24, 2004 #2


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    Are you familiar with redox reactions? Reduction and Oxidation. The cytoplasm of a cell is a reducing environment: an effect is that disulfide bonds within proteins get reduced and are thus hard to form.

    DTT is a reducing agent and often added to reducing buffers.
  4. Sep 24, 2004 #3
    thanks Monique! it seems like you know alot of things :smile: are you a PhD?
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