Reducing or not!

  • Thread starter mountain
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what is the difference between a non-reducing and a reducing buffer? :uhh:

and what does it mean with non-reducing and reducing?

thanks a bunch!
 
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  • #2
Monique
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Are you familiar with redox reactions? Reduction and Oxidation. The cytoplasm of a cell is a reducing environment: an effect is that disulfide bonds within proteins get reduced and are thus hard to form.

DTT is a reducing agent and often added to reducing buffers.
 
  • #3
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thanks Monique! it seems like you know alot of things :smile: are you a PhD?
 

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