Hi for biology we had to do a lab which we separated the plant pigments by chromatography of spinach. We used a solvent of 90% petroleum ether:10% acetone. After we did the experiment we ran out of time in class so the teacher went and recorded everyones results on there paper. The problem with this is we we got it back it was very faded, and only knew the general colour by what he circled, yellow or green. Then we had to calculate our Rf values. Now we are to compare them to the standard Rf values. I have not found all of these Rf values so if anyone knows what the standard Rf values are it would be much appreciated. Also on our chromatography paper the results of our Rf values are not very close to others on the internet. Also the colours that the teacher said appeared on the papers are not the same. Going from the solvent front to the origin the colours idicated are : yellow, yellow, yellow, green, yellow. I think the order of pigments is beta-carotene, lutein?, xanthophyll, chlorophyll a, and chlorophy b. But chlorophyll b is suppose to be olive green and what was idicated on our paper was yellow. The rest appear to seem right, order was but the Rf values seem to high for some. Any help would be much appreciated.