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RNAi screens for human cells

  1. Mar 28, 2004 #1


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    "RNA interference (RNAi) proved its worth in the lab as a powerful method of silencing gene activity in lower organisms such as Drosophila and C. elegans via the introduction of a long double-stranded RNA molecule. More recently, with small interfering and short hairpin RNAs (shRNAs) as the gene silencers, RNAi has begun to do the same job in mammalian cells. Two groups this week report the construction of RNAi libraries targeting thousands of human and mouse genes. They then go on to perform the first large-scale shRNA screens in mammalian systems. Paddison et al. prove the effectiveness of their library in a screen for human proteosome defects. And Berns et al. identify one known and five new components of the p53 signalling pathway in human cells."

  2. jcsd
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