Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

RT-pPCR products?

  1. Apr 13, 2005 #1
    I wonder should i sequence PCR products after i have run RT-qPCR? If not then how come i know that they are the right products?


    Thanks for any help.
     
  2. jcsd
  3. Apr 13, 2005 #2

    iansmith

    User Avatar
    Staff Emeritus
    Science Advisor
    Gold Member

    qPCR product is usuall not used for sequencing because the volume and the quantity of DNA is often insufficient and depending on the system you cannot pipette the reaction out of the tube. You could use you RT-PCR product.

    You should know it is the right product based on the primers and test done with these primer such as PCR and sequencing.
     
Know someone interested in this topic? Share this thread via Reddit, Google+, Twitter, or Facebook

Have something to add?



Similar Discussions: RT-pPCR products?
  1. About MMLV RT (Replies: 7)

  2. Rt- Pcr? (Replies: 4)

  3. Yogurt production (Replies: 9)

Loading...