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RT-pPCR products?

  1. Apr 13, 2005 #1
    I wonder should i sequence PCR products after i have run RT-qPCR? If not then how come i know that they are the right products?

    Thanks for any help.
  2. jcsd
  3. Apr 13, 2005 #2


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    qPCR product is usuall not used for sequencing because the volume and the quantity of DNA is often insufficient and depending on the system you cannot pipette the reaction out of the tube. You could use you RT-PCR product.

    You should know it is the right product based on the primers and test done with these primer such as PCR and sequencing.
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