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Running DNA and RNA through agarose gel electrophoresis

  1. Oct 6, 2009 #1
    1. The problem statement, all variables and given/known data

    How would you predict for samples of ssDNA and ssRNA (1kb long each) would run on agarose gel electrophoresis? Explain.

    2. Relevant equations



    3. The attempt at a solution

    From what i learned, RNA would run faster then DNA since RNA is more compact. Although what other factors are affecting it? Since the length of the two strands are the same, we can exclude it. Will the charge be any different, i.e the missing oxygen?
    Also, RNA can for hairpin loops that will affect its migration time. I believe that forming this loop will increase the speed since it is even compacted more.

    Any input with be appreciated!
    Thank You
    DoubleMint
    1. The problem statement, all variables and given/known data



    2. Relevant equations



    3. The attempt at a solution
     
  2. jcsd
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