What is the optimal light intensity for studying epithelial cells in microscopy?

[superwolf]

What is the approximate size of epithelia cells from the human oral cavity? And why must light intensity be low when studying them in bright field microscopy? (I know that phase contrast is better).

 

[Andy Resnick]

Cells obtained by a 'cheek swab' are large- 30 microns across, easily. Now, in vivo (and in cultured monolayers), the epithelial cells pack together and present a cross-section closer to 10 microns.

The intensity doen't need to be low, exactly- what is important is to filter out the IR part of the spectrum and prevent dry-out. Even so, too much light is damaging to cells- not cancer, but photodamage can occur.

 

[Blueshift5]

The size of epithelial cells can vary depending on their location in the body, but on average, epithelial cells from the human oral cavity can range from 20-200 micrometers in diameter. This size range allows them to form a protective barrier while also allowing for efficient absorption and secretion.

When studying epithelial cells in bright field microscopy, it is important to keep the light intensity low. This is because bright light can cause the cells to become overexposed, making it difficult to observe their structures and details. Additionally, bright light can also cause the cells to shrink and distort, making it challenging to accurately measure their size.

In contrast, phase contrast microscopy is a better option for studying epithelial cells as it enhances the contrast between the cells and their surrounding environment, allowing for a clearer and more detailed image. This technique also uses low light intensity, making it less likely to damage or distort the cells.

Overall, the size of epithelial cells in the human oral cavity is an important factor to consider when studying them under a microscope, and using the appropriate microscopy technique, such as phase contrast, can greatly improve the accuracy and quality of the observations.