Southern Blotting

  • Thread starter nokia8650
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  • #1
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Main Question or Discussion Point

In southern blotting, how are the DNA bands visualised? i have read two conflicting methods; one using ethidium bromide stain, and one using DNA probes. Can either be used, or are the two distinct methods used in different circumstances?

Thanks.
 

Answers and Replies

  • #2
atyy
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EtBr is just to visualise the DNA, and is non-specific for all DNA. The DNA probe allows you to detect a specific complementary base pair sequence.
 
  • #3
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Thanks so much for the reply; thats was what I thought logically made sense but I wanted to confirm it!
 

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