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Southern Blotting

  1. Mar 21, 2010 #1
    In southern blotting, how are the DNA bands visualised? i have read two conflicting methods; one using ethidium bromide stain, and one using DNA probes. Can either be used, or are the two distinct methods used in different circumstances?

    Thanks.
     
  2. jcsd
  3. Mar 21, 2010 #2

    atyy

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    EtBr is just to visualise the DNA, and is non-specific for all DNA. The DNA probe allows you to detect a specific complementary base pair sequence.
     
  4. Mar 21, 2010 #3
    Thanks so much for the reply; thats was what I thought logically made sense but I wanted to confirm it!
     
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