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Southern Blotting

  1. Mar 21, 2010 #1
    In southern blotting, how are the DNA bands visualised? i have read two conflicting methods; one using ethidium bromide stain, and one using DNA probes. Can either be used, or are the two distinct methods used in different circumstances?

  2. jcsd
  3. Mar 21, 2010 #2


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    EtBr is just to visualise the DNA, and is non-specific for all DNA. The DNA probe allows you to detect a specific complementary base pair sequence.
  4. Mar 21, 2010 #3
    Thanks so much for the reply; thats was what I thought logically made sense but I wanted to confirm it!
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