SV40 early region promoter (Psv)

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In summary, the SV40 promoter is a promoter from the SV40 virus DNA that can transcribe either early or late genes. The early region mRNA codes for the Large and Small Tumor Antigen Proteins, while the late region is expressed later and encodes the capsid proteins. The promoter is self-regulated to cause DNA replication once per cell cycle with the help of host cell proteins. The enhancer is most likely a host cell protein. This promoter is commonly found in plasmids used for fluorescence microscopy and can immortalize human cells.
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Monique
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What exactly is this promoter? As I understand it, it will only drive gene expression when you add an enhancer to it?
 
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The SV40 promoter is a promoter from the SV40 virus DNA. From its origin of replication it can transcribe either early or late genes. The early region mRNA codes for the Large and Small Tumor Antigen Proteins. Large T antigen binds DNA and acts like a helicase, recruiting the machinery needed to duplicate DNA. It binds the GAGGC in the SV40 origin and allows the virus to replicate. This is self regulated to cause DNA replication once per cell cycle because it needs DNA replication machinery from the cell to replicate. The late region is expressed later in time because it encodes the capsid proteins and is only needed when the virus is replicated and needs to encapsulate and leave the cell to infect other cells.
I'm not sure what the enhancer is but it most likely is a host cell protein.
 
  • #3
monique, are you using a new plasmid?

the ones I've used usually had both CMV and SV40 ori and promoters all over the place (for fluresence microscopy). The CMV promoter would point to the MCS, (GFP sequence before the MCS)and then followed by the SV40 poly A tail. The SV40 e promoter would point to the Kan/Neo resistance gene, follwed by HSV poly A and an optional thymidine kinase for double-selection/homologous recombination. I image there are many variations on this.

It is interesting to note that the T antigen of SV40 will immortalize a human cell. Infact, I have used such a transformed cell line (SVG).
 

Related to SV40 early region promoter (Psv)

1. What is the SV40 early region promoter (Psv)?

The SV40 early region promoter, also known as Psv, is a regulatory sequence found in the genome of the simian virus 40 (SV40). It controls the expression of early viral genes, which are necessary for viral replication and infection.

2. How does Psv function?

Psv functions by binding to specific transcription factors, which then recruit RNA polymerase to the promoter sequence. This initiates the transcription of early viral genes, allowing the virus to replicate and spread.

3. Can Psv be used in gene expression studies?

Yes, Psv has been extensively studied and is commonly used as a promoter sequence in gene expression studies. It is known to be a strong and efficient promoter, making it a valuable tool for researchers.

4. Are there any human diseases associated with Psv?

There is currently no evidence to suggest that Psv is directly associated with any human diseases. However, since the SV40 virus has been linked to some cancers in animal studies, there have been concerns about the potential risks of using Psv in gene therapy.

5. What is the significance of Psv in biotechnology?

Psv has played a significant role in biotechnology, particularly in the development of recombinant DNA technology. Its strong promoter activity and ability to drive high levels of gene expression have made it a valuable tool for producing proteins of interest in various biotechnological applications.

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