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The limitations of RP-HPLC

  1. Aug 29, 2009 #1
    As HPLC is such a common method in chemical analysis, I was just wondering what are the limitations to such a technique? What compounds/mixtures is it limited to analyzing?
     
  2. jcsd
  3. Sep 5, 2009 #2

    chemisttree

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    Well it certainly is limited to the analysis of compounds which are soluble in the mobile phase. It is limited to the analysis of compounds which are not reactive with the mobile phase or stationary phase as well.
     
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