Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

Thin-layer chromatography

  1. Feb 20, 2007 #1
    Is it possible to separate cis/trans isomers of diazo compound using Thin-layer chromatography?
  2. jcsd
  3. Feb 20, 2007 #2
    It would probably be difficult to seperate those compounds by TLC due to their similarity in structure and the choice limitations one has with TLC as well as collecting the sample once it has seperated. A similar technique would be liquid chromatography. Packing the column with an appropriate stationary phase (most often silica gel) and using an appropriate mobile phase the compounds might seperate.

    The mobile phase choice will depend on the polarity of your compounds. Since they are diazo compounds they are fairly polar. If you have acetonitrile you could try a mobile phase of 70:30 acetonitrile/water. If you want to increase the speed of the seperation the column can be pressurized. Depending the the seperation you will have to change the mobile phase polarity by switching solvents or changing the percentages. Most likely the mobile phase will have to become less polar to encourage the isomers to spend more time in the stationary phase.

    If the compounds are too close in nature it will be difficult to seperate them without using more complicated High Performance LC equipment.
  4. Feb 20, 2007 #3
    I just want to know, is it theoretically possible?
  5. Feb 20, 2007 #4
    theoretically with the correct stationary phase and mobile phase and if the isomers have slightly different chemical properties, then yes.
Share this great discussion with others via Reddit, Google+, Twitter, or Facebook