1. Not finding help here? Sign up for a free 30min tutor trial with Chegg Tutors
    Dismiss Notice
Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

Thin Layer Chromatography

  1. Feb 1, 2008 #1
    1. The problem statement, all variables and given/known data

    How does heating affect the spots on the TLC?

    2. Relevant equations


    3. The attempt at a solution

    I have no clue, unless heating evaporates the solution and therefore the spots disappear?
    or Perhaps the elutent evaporated due to heat rather then the solution?
    Last edited: Feb 1, 2008
  2. jcsd
  3. Feb 2, 2008 #2
    Heating may or maynot affect the components of the organic solvents which have been adsorbed in the adsorbent.

    however, if the temp is high enough, then the solvent may vaporize.
    Last edited: Feb 2, 2008
  4. Feb 2, 2008 #3


    User Avatar
    Homework Helper
    Education Advisor
    Gold Member

    The higher temperature may (just a guess) make the sample spot become messier because of the increased random motion of the molecules of solvent. If this is correct, then maybe someone can transcribe into more precise language.
  5. Feb 4, 2008 #4


    User Avatar
    Science Advisor
    Homework Helper
    Gold Member

    Is this question being asked in context with another question? Heating a TLC is performed sometimes after treatment with H2SO4. Did you treat the TLC with H2SO4?
  6. Feb 4, 2008 #5
    yes you are correct , chemisttree

    sorry about that
  7. Feb 5, 2008 #6


    User Avatar
    Science Advisor
    Homework Helper
    Gold Member

    It's called visulization by charring. The sulfuric acid will catalyze the charring of organic compounds at elevated temperatures (~120C).
    Last edited: Feb 6, 2008
Know someone interested in this topic? Share this thread via Reddit, Google+, Twitter, or Facebook

Have something to add?

Similar Discussions: Thin Layer Chromatography
  1. Paper chromatography (Replies: 3)

  2. Ion Chromatography (Replies: 1)

  3. Paper Chromatography (Replies: 1)