Hello everyone, I was poor on TLC before, and am poor now I want to ask a question to you guys. Please look at the attached zip file with a tif image. My question is very simple: Is it possible to fully isolate the impurities (yellow spot and light olive-mustard-colored streaking) from my ester, which is immobilized on the start point? I have very poor knowledge on chromatography, and I have a limited amount of ester sample. I don't want to make a mistake while performing the column chromatography. More simply, can I avoid the streaking from involving my ester sample and drive off from the column completely? If you want to know, thf alone elutes my ester with elution solvent (Rf is about 1). thf:MeOH mixtures cause some streaking effects. I am planning to isolate the impurities by eluting through the column with MeOH first to drive off the yellow spot, and then employing 1:1 MeOH:thf mixture to isolate the mustard-colored streaking, and after these have gone, eluting with thf alone to obtain my purified ester sample. Thank you for your time on even reading this post.