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Tm of a PCR product?

  1. Apr 16, 2005 #1
    I know how to calculate Tm of a primer but what about of a PCR product?

    I want to run the melting curve in RT-qPCR to test for the products and i want to know Tm of the products to be sure.


    Thanks.
     
  2. jcsd
  3. Apr 17, 2005 #2

    Monique

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    Why not use the same calculation as the Tm of a primer? A good calculator should takes into account the length of the nucleic acid. How do you calculate it now?
     
  4. Apr 17, 2005 #3

    Monique

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  5. Apr 17, 2005 #4

    The difference between a primer and a PCR product is that the PCR product is a double stranded DNA while the primer is just a singel strand. So should i calculate Tm for the PCR product like as it is a single strand?
     
  6. Apr 17, 2005 #5

    iansmith

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    Although you calculate Tm with a single strand for primer, it is use to seperate your double stranded DNA.

    So for your PCR product calculate with the single stranded DNA sequence.
     
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