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Homework Help: Transcriptional vs. translational fusion

  1. May 6, 2010 #1
    What is the difference between transcriptional and translational fusion?

    As I understand it, transcriptional fusion places a reporter, like lacZ, downstream of the promoter of your gene of interest (so it does not include your entire gene). Is this the same thing in a translational fusion?

    What data does translational fusion give you that transcriptional fusion doesn't? The way I have come to understand it, the transcriptional fusion will still allow for B-gal to be translated and then you can measure its activity to determine relative transcription levels.... is this anywhere near correct?


    thanks
     
  2. jcsd
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