Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

Transfection question

  1. Jul 1, 2005 #1


    User Avatar

    i was just wondering if anybody out there had a good guess as to how many HeLa cells were on a 100% confluent, say, 100mm plate (any size would do). my guess would be between 750,000 to 2 million? depending on how dense they get. what would you think 70% would be? does anyone have any numbers for plates this confluency that were counted/sorted?
  2. jcsd
  3. Jul 2, 2005 #2
    there is no way to give a reliable number.

    there is just too much variation between cell lines (even if they are all HeLa cells), how many times the cells have been passaged, and your particular growth conditions.

    you just have to get down and dirty and count them. you can do it the traditional way with a foot tapper and microscope, or there are computer programs that will count pixels for you.

    if you are suspecting contamination, it should be evident if you examine the plates daily.
Share this great discussion with others via Reddit, Google+, Twitter, or Facebook

Similar Threads for Transfection question Date
Neuron parts identification question Apr 15, 2018
Disc hernia question Mar 10, 2018
Gene Manipulation / Transformation Dec 15, 2017
Transfection-Tissue culture Mar 19, 2005
Transfection/ transformation Nov 13, 2003