HeLa Cell Counts for 100% Confluent Plates

[eab]

i was just wondering if anybody out there had a good guess as to how many HeLa cells were on a 100% confluent, say, 100mm plate (any size would do). my guess would be between 750,000 to 2 million? depending on how dense they get. what would you think 70% would be? does anyone have any numbers for plates this confluency that were counted/sorted?

 

[quetzalcoatl9]

i was just wondering if anybody out there had a good guess as to how many HeLa cells were on a 100% confluent, say, 100mm plate (any size would do). my guess would be between 750,000 to 2 million? depending on how dense they get. what would you think 70% would be? does anyone have any numbers for plates this confluency that were counted/sorted?

there is no way to give a reliable number.

there is just too much variation between cell lines (even if they are all HeLa cells), how many times the cells have been passaged, and your particular growth conditions.

you just have to get down and dirty and count them. you can do it the traditional way with a foot tapper and microscope, or there are computer programs that will count pixels for you.

if you are suspecting contamination, it should be evident if you examine the plates daily.

 

[ravenprp]

It is difficult to give an accurate estimate without knowing the specific conditions and methods used for culturing the HeLa cells. However, based on previous studies, a 100% confluent 100mm plate could contain anywhere from 750,000 to 2 million cells, as you mentioned. The actual number may vary depending on the growth rate and density of the cells. As for a 70% confluent plate, it could potentially have around 500,000 to 1.4 million cells. It would be helpful to have more information on the specific techniques and protocols used for culturing the cells in order to provide a more precise estimate. Perhaps someone with experience in working with HeLa cells at different confluency levels could share their observations and numbers for comparison.