Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

Transformation Effiency (please help)

  1. May 2, 2005 #1
    I know that the formula to calculate the TE is

    (#of transformants)/(g of DNA) * (Final volume at recovery) / (Volume plated)

    but what exactly is "fianl volume at recovery"? Do I calculate the total # of pGal DNA, X-gal and recovery broth that I mixed up?????
  2. jcsd
  3. May 2, 2005 #2


    User Avatar
    Staff Emeritus
    Science Advisor
    Gold Member

    You need that to calculate what the fraction was that you plated. Did you plate all your transformation volume, or did you only plate part of it?
Share this great discussion with others via Reddit, Google+, Twitter, or Facebook