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Transformation Effiency (please help)

  1. May 2, 2005 #1
    I know that the formula to calculate the TE is

    (#of transformants)/(g of DNA) * (Final volume at recovery) / (Volume plated)

    but what exactly is "fianl volume at recovery"? Do I calculate the total # of pGal DNA, X-gal and recovery broth that I mixed up?????
     
  2. jcsd
  3. May 2, 2005 #2

    Monique

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    You need that to calculate what the fraction was that you plated. Did you plate all your transformation volume, or did you only plate part of it?
     
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