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Transformation of bacterial cell

  1. Dec 1, 2007 #1
    does any one know how to calculate the Transformation efficiency?
  2. jcsd
  3. Dec 2, 2007 #2


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  4. Dec 2, 2007 #3
    actually I kind of have the formula of the transformation efficiency

    colonies on plate/ amount of DNA plated in (ug)

    I just don't know how to find the amounf of DNA plated..... well i kind of have an answer
    but it's really small..... but this website saids that the the transformation efficiency should be a big number.... something like 10^9 that they got from their example........

    so, i don't know if i am doing it right.......
  5. Dec 2, 2007 #4
    or maybe it's because my experient didn't work out that great
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