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Unexpected Enviromental factors On Enzyme Activity?

  1. Mar 21, 2004 #1
    Hi all,

    I am currently a college frosh taking cellular biology lab. In lab we did an experminet to measure how tempature effects enzyme activity. We put 1 mL of B-galactosidase (when a certain substrate is added to this enzyme, a yellow liquid (product) is formed). Then we use a Spec-20 to measure the absorbance of light at 420 nm, thus we can measure the rate of the reaction).

    I know that a graph of enzyme activity (rate) vs. temperature should produces an upside down possion distributin ( or a funky "U" shape) This beacasue enzymes have a peak tempature at which they work best at.

    On my graph, the curve contains two peaks, one at 37 degree (body temp) and the other at 60 degrees.

    Can an enzyme have two peak levels of effcintcy. It does make sense to me. I thought that the rate at which enzymes process substrate increase with temaperature untill the tempature is high enough to denature the enzyme rendering it inactive.

    Thank your the help, it is greatly apperated
  2. jcsd
  3. Mar 22, 2004 #2
    At that temp,the enzyme should be denatured and completely useless. Could something else have caused the peak???

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