UV visible spectrometry is a scientific technique used to measure the amount of light absorbed by a substance at different wavelengths in the ultraviolet (UV) and visible (visible) regions of the electromagnetic spectrum. It is commonly used in chemistry, biochemistry, and other fields to identify and quantify the chemical components of a sample.
UV visible spectrometry works by passing a beam of light through a sample and measuring the amount of light that is absorbed at different wavelengths. This absorption of light is caused by the interaction of the sample's molecules with the specific wavelengths of light. The resulting absorption spectrum can then be used to identify and quantify the components of the sample.
UV light has a shorter wavelength and higher energy than visible light. UV light is not visible to the human eye, while visible light is the range of wavelengths that can be seen by the human eye. In UV visible spectrometry, both UV and visible light are used to analyze a sample, with UV light being used for more energetic and complex molecules and visible light for simpler molecules.
UV visible spectrometry has a wide range of applications, including identifying and quantifying chemical components in a sample, determining the concentration of a substance in a solution, and studying the structure and properties of molecules. It is commonly used in fields such as chemistry, biochemistry, environmental science, and pharmaceuticals.
There are several advantages to using UV visible spectrometry. It is a non-destructive and non-invasive technique, meaning the sample can be recovered and used for further analysis. It is also a relatively quick and simple method, with results available in a matter of minutes. Additionally, it is a highly sensitive technique, able to detect small changes in concentration and suitable for analyzing a wide range of substances.