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Weight to pmol/l for nucleotides

  1. Apr 11, 2005 #1
    How can i transform weight into pmol/l when i have to prepare the primers?

    Which formula should i use to know this transformation?

    The protocol; Dissolve dried primer stocks at 100 pmol/l in RNAse-free water and store at -20°C.

  2. jcsd
  3. Apr 11, 2005 #2


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    You need to know the sequence of your primers, then it's just basic chemistry where you calculate your molecular weight to determine molarity.
  4. Apr 11, 2005 #3
    What if my primer has 6 nucleotides do you know how i can calculate into 100 pmol/l?

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