We're attempting to measure the immunogenic affects of a particular molecule after subsequent acid and base treatments. As a first version, we're keeping it simple. After all treatment and neutralization, we wind up with 400 uL 0.2 N HCl + 400 uL 0.2 N NaOH + 200 uL H2O. We are getting massive precipitation upon neutralization! To test whether this was due to our compound, I did a water control and the same thing happened. Considering the solubility of NaCl is in excess of 6 M, what the hell could be going on? Any ideas would be greatly appreciated. This is basic gen chem stuff, so I am baffled.