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Western Blot Two Proteins

  1. Aug 31, 2011 #1
    Hello Forum,

    please have a look at this image

    http://imageshack.us/photo/my-images/98/0bigg.jpg/

    There are two proteins A and B in the right column.
    Marker B is the protein´s length of healthy people.
    But as you can see there are two different markers for this protein (the western Blot was made for one patient).

    How can you explain the appearance of two different markers in one patient?

    Can you explain with examples how changes of genes can lead to this result?
     
  2. jcsd
  3. Aug 31, 2011 #2

    Andy Resnick

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    This sounds like a homework problem. I can think of several explanations, what do you think could have happened?
     
  4. Aug 31, 2011 #3
    Can you explain with examples how changes of genes can lead to this result?

    The answer to this question is a nonsense-mutation because of the substitution of one base-pair (exmaple 1). The second example is a nonsense-mutation because of insertion or deletion of base-pairs.

    Do you have any further examples which explains this result?

    How can you explain the appearance of two different markers in one patient?

    The different genes express two different proteins. The "healthy" gene codes for the "healthy" protein and the other gene codes for the short protein. Both proteins are expressed in this patient.
    Mutation is caused due to radioactive waves or it is inherited. It is possible that there is a co-dominant expression and, therefore, both proteins are expressed.

    Do you have any other possibilities?

    The markers above are related to the protein Enolase. Marker B is the Enolase and marker A is the mutated enzyme.

    Maybe you have some more accurate possibilities considering the mutations and their causes which are related to this enzyme.
     
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