Okey, i am confused about this matter. I have two different samples. One is 5 untreated/control samples (same kind) and the other is a 50 treated samples (same kind). I wonder what kind of statistic methods i can use to compare the results between them? I don't think that i can use ANOVA, since they need at least 3 different samples. Besides i can not use T-test or Z-test either, since the former needs both samples to be at a small number n<30, while the latter needs both samples to be large n>30. My samples is n=5 (controll samples) and n=50 (treated samples). Hope for inputs. Thank you!