Anticancer Res. 2003 Mar-Apr;23(2B):1405-9.
Suicide gene therapy using keratin 19 enhancer and promoter in malignant mesothelioma cells.
Ishiwata N, Inase N, Fujie T, Tamaoka M, Yoshizawa Y.
Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8519, Japan.
To achieve the specific expression of a transfected suicide gene in malignant mesothelioma cells, we applied the enhancer-promoter fusion sequence of the keratin 19 (K19) gene. Northern blot analysis of three mesothelioma cell lines demonstrated that K19 mRNA was expressed most abundantly in the H2052 mesothelioma cell line. Subsequently, in a luciferase reporting assay, K19 promoter (260 bp) exhibited higher promoter activity in H2052 cells than in the other two cell lines. In addition, ligation of a 3' enhancer (80 bp) of the K19 gene to upstream of the K19 promoter sufficiently enhanced the promoter activity. After transfecting an expression vector containing the K19 enhancer-promoter bound thymidine kinase gene (pK19-TK) into the H2052 cells, the pK19-TK transfected cells became more sensitive to GCV than non-transfected cells in vitro and in vivo. In a mouse peritoneal model of malignant mesothelioma, in vivo transfection of pK19-TK by cationic liposome and systemic administration of GCV inhibited the growth of peritoneal tumors. The K19 enhancer-promoter sequence seemed to be specific and efficient enough for the expression of the transfected suicide gene in malignant mesothelioma cells. Anticancer Res. 2003 Mar-Apr;23(2B):1405-9.
Mesothelioma is a rare and aggressive form of cancer that affects the lining of the lungs, heart, or abdomen. It is primarily caused by exposure to asbestos, a mineral that was commonly used in construction and manufacturing industries.
Law firms are interested in Mesothelioma cases because they believe that companies who used asbestos knew about its health risks and failed to warn and protect their employees. This has led to numerous lawsuits and large settlements for victims and their families.
The most common symptoms of Mesothelioma include chest pain, shortness of breath, fatigue, and weight loss. These symptoms often do not appear until the cancer has progressed to a later stage, making early detection and treatment difficult.
Mesothelioma is diagnosed through a combination of imaging tests, such as X-rays and CT scans, and a biopsy of the affected tissue. It is important to seek medical attention if you have a history of asbestos exposure and experience symptoms that may be related to Mesothelioma.
While there is currently no cure for Mesothelioma, treatment options such as surgery, chemotherapy, and radiation therapy can help manage symptoms and improve quality of life. Early detection and treatment can also improve the chances of survival.