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Whole cell patch clamp

  1. May 19, 2006 #1
    I don't really know the principles of this technique despite doing some reading:

    the cell patch is destroyed, and the contents of the cytoplasm can mix with the contents of the electrode.

    there is a low resistance pathway - so what?

    and how can measurements be made if the membrane is destroyed?
  2. jcsd
  3. May 19, 2006 #2
    The entire cell membrane is not destroyed, only the membrane at the interface of the tip/cell is made porous. Because of that measurements can be made using this particular technique. You should do a quick search on google and you'll be able to find movies/animations that demonstrate and explain this.

    Hope that helps.
  4. Oct 13, 2008 #3
    Dear Colleagues,
    Is it possible to study membrane potential - intracellular K+ (Na+) content relationship by whole cell patch-clamp method? Some article please...
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