Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

Whole cell patch clamp

  1. May 19, 2006 #1
    I don't really know the principles of this technique despite doing some reading:

    the cell patch is destroyed, and the contents of the cytoplasm can mix with the contents of the electrode.

    there is a low resistance pathway - so what?

    and how can measurements be made if the membrane is destroyed?
     
  2. jcsd
  3. May 19, 2006 #2
    The entire cell membrane is not destroyed, only the membrane at the interface of the tip/cell is made porous. Because of that measurements can be made using this particular technique. You should do a quick search on google and you'll be able to find movies/animations that demonstrate and explain this.

    Hope that helps.
     
  4. Oct 13, 2008 #3
    Dear Colleagues,
    Is it possible to study membrane potential - intracellular K+ (Na+) content relationship by whole cell patch-clamp method? Some article please...
     
Know someone interested in this topic? Share this thread via Reddit, Google+, Twitter, or Facebook

Have something to add?



Similar Discussions: Whole cell patch clamp
Loading...