Recent content by fleetwood99

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    How long to centrifuge at 3,000xg instead of 5,000xg for RNA purification?

    I've been purifying RNA for RT-PCR cloning. I have been using the same type of kit (Promega Total RNA Kit). My 260/280 ratio has been between 1.9-2.1, which is good. However I just ran a new set of samples and the ratio is down to 1.7 which isn't so great. This might mean I have a lot of DNA...