Recent content by hlaurenc

I knew that :) :) :)

Thanks for your reply - much appreciated. 1) we did not perform a negative control, no. This may be a silly question: how would hydrolysis occur without the presence of an enzyme? Surely, they'd be no reaction. 2) thanks - noted. Just for information - the below figures are umol of product...

I recently carried out an investigation into the specificity of chymotrypsin and trypsin which I am in the process of writing up. In short, combined substrate with enzyme, incubated for 15 mins and measured at 430nm and used trendline equation to determine umol products formed per minute...