Recent content by Jiayun

Yeah I am aware of the single spot illumination in confocal microscopy. I am wondering whether deconvolution increases the resolution for confocal images.

I see. This makes sense. Thank you very much

Oh yes I was thinking of Gaussian when actually it is supposed to be Airy disk :sorry: I am guessing PSF in practical situations are not Airy disks since deconvolution is shown to improve resolutions? So basically deconvolution increases resolution by amplifying high frequency components?

I see, so basically we can't get infinite solution even theoretically due to the zeros in the Airy disk? Does this mean theoretically deconvolution doesn't help diffraction limited system since they already reach the highest spatial frequency allowed by the first Airy disk? Or deconvolution can...

The widely recognized theory for the detected image in microscope (I believe) is calculated by taking the convolution of the object and a point spread function (PSF). Deconvolution tries to reverse this operation to get back the original object. This explanation sounds easy and intuitive but...