We have used two programs to predict the transcription factor binding sites with setting the similarity score as 85%, and found that 48 TFs can bind to the DNA sequence. Theremore, we worry whether there are other cofactor not directly binding to the promoter, that affect the expression of the gene.
We cloned different length segments of the promoter, and linked the segments to luciferase reporter gene in expression vector, and transfected the vectors into A and B cell lines. Finally, we found that the segments with the length of >=250 bp have high activity in A cell line and no activity...
We have been doing research on the differential expression of one gene between two cell lines (A and B cell lines), with high expression in A cell line and no expression in B cell line. We found that ~250 bp in the promoter of the gene has its activity in A cell line, but no activity in B cell...